LOCUS       40924_35858        7857 bp DNA     circular SYN 18-DEC-2018
DEFINITION  P-element cloning system vector pP{CaSpeR4-lo+}, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Roman G, Endo K, Zong L, Davis RL.
  TITLE     P[Switch], a system for spatial and temporal control of gene 
            expression in Drosophila melanogaster
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 98 (22), 12602-12607 (2001)
  PUBMED    11675496
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Roman G, Davis RL.
  TITLE     Conditional expression of UAS-transgenes in the adult eye with a new
            gene-switch vector system
  JOURNAL   Genesis 34 (1-2), 127-131 (2002)
  PUBMED    12324966
REFERENCE   3  (bases 1 to 7857)
  AUTHORS   Roman G, Davis RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2002) Molecular and Cellular Biology, Baylor 
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   4  (bases 1 to 7857)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7857)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2001"; volume: "98"; issue: "22"; pages: 
            "12602-12607"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Genesis 34 
            (1-2), 127-131 (2002)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-2002) Molecular and Cellular Biology, Baylor College of 
            Medicine, One Baylor Plaza, Houston, TX 77030, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..586
                     /label=P element 5' end
     gene            complement(597..4733)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    complement(4739..4772)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4865..5097
                     /label=P element 3' end
                     /note="P element 3' end"
     misc_feature    5098..5598
                     /label=white linker sequence
                     /note="white linker sequence"
     promoter        5899..6003
                     /label=AmpR promoter
     CDS             6004..6861
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7035..7623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7629..7857
                     /label=white linker sequence
                     /note="white linker sequence"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1381 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1441 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1501 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1561 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1621 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1681 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1741 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1801 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1861 gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1921 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1981 aaatcgaatc gattcattag aaagttagta aattattaat atgcaaatgt attctaaaca
     2041 agacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     2101 atggctccgt tgatattcat cacgcccact tgcgtgagtt gttggcccaa aaagatgagg
     2161 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcat
     2221 atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt ttctgcaaaa ctataaacta
     2281 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctaa ctaaaatcta
     2341 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     2401 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     2461 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     2521 cggctgctcc agtggcttct ccaaattttt ggtggccaac aactgctcca tatcccgggc
     2581 tactttgcta atagcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     2641 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     2701 acactgggca cccacgctgg ataggagttg agatgtaatg taatgctaga tacccttaat
     2761 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     2821 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     2881 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     2941 cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     3001 gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     3061 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     3121 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     3181 ccatggcctg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     3241 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     3301 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     3361 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     3421 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     3481 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     3541 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     3601 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     3661 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggcagga gcgtgccgta
     3721 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     3781 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     3841 aaaacgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat
     3901 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatgtagtcg aaaatgaatg
     3961 cctttagatg tcttgatcat gatatgatct taaaaattgt cttatatagc gagcacagct
     4021 accagaataa tctgtttcgt gtcactattt gtttgtgcga ttgcggtttg ggatttttgt
     4081 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa
     4141 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt
     4201 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc
     4261 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa
     4321 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa
     4381 ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa ctgaaggcgg acattgacgc
     4441 taggtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg
     4501 atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg
     4561 agtgagaggt aatcgaaaga acctggtact tcaaataccc ttggatcgaa gtaaatttaa
     4621 aactgatcag ataagttcaa tgatgtccag tgcagtaaaa aataaaaaaa aaatatgttt
     4681 ttttaaatct acattctcca aaaaagggtt ttattaactt acatacatac tagaattcat
     4741 aacttcgtat agcatacatt atacgaagtt ataattcggt acccgcccgg ggatcagatc
     4801 cgcggccgca taggccacta gtggatctgg atcctctaga gtcgacctgc aggcatgcaa
     4861 gctttgcgta ctcgcaaatt attaaaaata aaactttaaa aataatttcg tctaattaat
     4921 attatgagtt aattcaaacc ccacggacat gctaagggtt aatcaacaat catatcgctg
     4981 tctcactcag actcaatacg acactcagaa tactattcct ttcactcgca cttattgcaa
     5041 gcatacgtta agtggatgtc tcttgccgac gggaccacct tatgttattt catcatggtc
     5101 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     5161 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt ttaccattat
     5221 taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca
     5281 ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt
     5341 agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa
     5401 ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg
     5461 cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc
     5521 tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt
     5581 tagttccgcg cgattcggtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     5641 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     5701 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5761 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     5821 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5881 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5941 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6001 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6061 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6121 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6181 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6241 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6301 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6361 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6421 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6481 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6541 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6601 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6661 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6721 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6781 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6841 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6901 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6961 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7021 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7081 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7141 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7201 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7261 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7321 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7381 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     7441 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7501 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7561 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7621 aaaaacgcct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
     7681 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
     7741 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
     7801 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggcc
//
